Vivaspin ® 20 centrifugal concentrators offer increased volume flexibility and performance to the Vivaspin ® potfolio. Vivaspin ® 20 handles up to 20 ml in swing bucket rotors and 14 ml in fixed angle rotors accepting 50 ml centrifuge tubes. Vivaspin ® 20 features twin vertical membranes for unparalleled filtration speed and 100-fold concentration. Volume graduation allows monitoring the concentration process and the modified dead stop pocket simplifies pipetting of the final concentrate. For higher process flexibility, Vivaspin ® 20 is available with unique accessories and operating methods.

• Amicon Filter
• Centriprep Ym 30

For one-step desalting following concentration, a diafiltration cup filled with buffer is can be placed on the concentrator and spun another time to achieve 98% salt removal, instead of two spins to achieve the same result with the traditional refill and re-spin procedure. When an appropriate centrifuge is unavailable, or for single sample processing, for gas pressure filtration, Vivaspin ® 20 can be filled with up to 15ml and then pressurised for bench top concentration. For even faster processing, gas pressure filtration can be combined with centrifugation. 'Pressure-fugation' is particularly useful for difficult or viscous samples such as serum, when using a low process temperature, or generally when minimum process time is essential. High flux PES membrane. High retentate recovery 90%. Vertical twin membrane for ultra-fast concentration.

Universal rotor compatibility. Safe sample concentration due to dead-stop volume. Convenient concentrate recovery by pipetting. Volume graduation for easy reading/monitoring. 100-fold concentration with twin vertical membranes. Additional option for pressurized operation Rotor Compatibility Please note: Vivaspin ® 20 (30 mm x 116 mm) is designed to fit into rotors that can accommodate Falcon 50 ml conical bottom tubes, e.g. Beckman Allegra 25R with TS-5.1-500 swing-out rotor with BUC 5 buckets and 368327 adaptors; Beckman TA-10.250 25° fixed angle rotor with 356966 adaptors; Heraeus Multifuge 3 S-R with (Heraeus/Sorvall) 75006445 swing out rotor with 75006441 buckets and adaptors for Falcon 50 ml conical bottom tubes.

These devices are not designed to fit into rotors that only accept round bottom 29 mm x 105 mm tubes, e.g. Sorvall SS34 or Beckmann JA 20. If your rotor accepts only 29 mm x 105 mm round bottom tubes, please use the Vivaspin ® Turbo 15, which can be used in either round bottom or conical centrifuge tubes Performance Characteristics Time to concentrate up to 30x min. Splinter cell conviction russian to english patch 2017. at 20°C and solute recovery% Mode Centrifuge Centrifuge Bench top Press-fuge Rotor Swing bucket 25° Fixed angle Pressure Swing bucket Sart volume 20 ml 14 ml 10 ml 10 ml Min./Rec.

Adam, We reuse concentrators but only for the same protein. Don't cross-contaminate) I believe we got a protocol from the centricon manual or the Amicon catalog. At any rate we soak the filter units in 70% ethanol and never let them dry.

Amicon Filter

Just before reusing them we rinse them and spin with the same buffer in which our protein is solubilized. PBS) Then we load on the new protein and spin. Hope this helps. It seems to work fine for us. Of Medicine Univ. Of Alabama at Birmingham P.S.

I know you from Steve's lab I think. In article (Anne E. Hall) writes: Hi- I have re-used the centricon 30's. I don't know just how long you can reuse them, but I have successfully re-used them up to four times.

Centriprep Ym 30

In between uses I store them with 70% EtOH and wrap them with parafilm and keep them in the fridge. That's interesting. We have tried to store centripreps in water:EtOH and then found that they no longer worked properly. I figure the filter material is the same in the centricons and centripreps. We store ours in water with a little sodium azide - seems to work fine. Frances Chen Univ.CA at San Diego.